Origin

In October 2009, the live video game streaming site DopefishLives posted a clip from their playthrough of Call of Juarez: Bound in Blood in which the player came across a goat in the game. A glitch caused the goat to levitate towards the sky when the player got too close to it. In this instance, the player backed the goat up against a ladder, making it seem like the goat was climbing it.

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The player's reaction to the ladder-climbing goat led to YouTube users remixing the audio with other videos:

There are also other audio remixes and response videos, including ones where viewers tried not to laugh while watching the video.