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It is unknown where the Malleo meme and its vectorised sprites surfaced in the web. However; the sprite pre-dates to the DOS version of the educational game Mario Is Missing! . The sprite is seen in the intro and ending cutscenes of the game. The intro cutscene is provided below:
The above picture is Malleo’s original non-pixel sprite, before the new vectorized version. This version became widely popular over 4chan. As well as many other forums over the years. These changes can be seen in some of the 4chan archives .
4chan Archives – http://chanarchive.org/4chan/v/21407/ms-paint-your-favourite-reaction-image-v-here-s-mine