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“Nyeh” comes from “Yu-gi-oh the abridged series” with one of the characters, Joey Wheeler, says in confusion, “Nyeh?” The show was created by famous internet flash creator “Little Kuriboh.”
Ever since, Joey has been saying Nyeh throughout season 2 of the show. It has so far been involved in many YouTube videos.
It is most notable in the show, but has had it’s popularity boosted up from his appearence of “Joey rolling” people on the video Re: NTAS CAsting Video!.