My Little Pony: Friendship is Magic - Image #222,449

Let's see:

Loony Luna, Pinkamina Diane Pie, Li'l Miss Rarity, Ruby Pinch, Berry Punch, Dr. Colgate, Surprise,
Molestia, Pinkie Pie Solutions, Raindrops, Human Luna, Friendly Twilight, Futashy, Firefly,
Stalkerloo, Velvet, Filly Twilight, My Little Chubbies (three are shown), and Woonastuck.

The ones I didn't get in one try are:
Dash's Colgate, Cteno, and Lady Octavia (I found the answers on Raindrops' Tumblr).

Lol, Scootablock on Futashy. :P