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Yay! / Fluttershy's Cheer is a popular remark form the popular television show: My Little Pony: Friendship is Magic. It has also been done by other notable characters such as Team Fortress 2 (My Little Fortress 2).
The phrase first came from Episode 16 of Season 1: "Sonic Rainboom". In the beginning of the episode, Rainbow Dash is teaching Fluttershy how to cheer properly. However, when trying it out, the saying is soft, not requiring up to Rainbow's standards. After trying again repeatedly and one last one with a huge amount of air, it still comes out the same. The scene ends with Rainbow Dash falling backwards and Fluttershy saying: "Too Loud?" confused in the problem of her last cheer.
Other included phrases in the show
This one however was used in the episode
The remark has been known as the cutest scene from the show itself. The phrase has been used in YTPMV's, reenacted and remembered of most of all throughout the series making related videos gain hundreds and thousands of views.
Avast Fluttershy's Ass – 20% Cooler Yay Equaliser Edition – 700,641 views
Fluttershy's Cheer (Colt Version) – 42,865 views
MLP Cosplay Video: Fluttershy's Cheer – 105,526 views
Ze spy attempts a Fluttershy fandub. – 17,179 views