Notes:

Source
Thanks Danbooru for the translations!

Part 1(Right)
Wriggle: Applejack, why are you sad?
Applejack: New audiences mistook me for a boy again.

Applejack: I'm a teenage girl just like the others…
Wriggle: I see, i am always mistaken for a boy as well. As a girl, i do feel your pain.

Applejack:Yeah, you boys… WHAT?! YOU ARE A GIRL?!

Applejack: I didn't mean it… I mean, you look like a boy… Uhh… No, no no no, i mean i didn't realize that… Sorry~ my mistake…

Part 2(Left)
Minoriko: Applejack, feeling down?

Applejack: Despite being one of the six protagonists, i always have little popularity. Even in this 4koma! I got my appearance in ep.9!

Unpopular sisters x2