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The Twilight Sparkle Scepter, also known as the Twilicane, is a pure gold scepter topped with the head of the My Little Pony: Friendship is Magic character Twilight Sparkle wearing a sheepish grin.
In Season 4 Episode 2 of My Little Pony: Friendship is Magic, originally aired on November 23rd, 2013, the villain character Discord gives Twilight Sparkle a scepter and a royal cape while mocking her for being a princess after leaving her friends in the Everfree Forest (shown below).
After the episode aired on November 23rd, 2013, threads about it began to appear on 4chan’s /mlp/ (pony) board, with the scepter first being pointed out a few hours later in a screencapture thread. The first seperate thread about the scepter was launched a few hours after that, quickly resulting in image macros and photoshops of the scepter.
On November 24th, the My Little Pony: Friendship is Magic storyboard artist Sabrina Alberghetti posted a tweet linking to a face swapped image of Twilight Sparkle and the scepter. That same day, Alberghetti also commented on a vector of the scepter uploaded to DeviantART, saying that it was she who animated that scene.
So apparently this is a thing now? You guys are nuts. http://t.co/VIH3f4vNrU— Sabrina Alberghetti (@TheRealSibsy) November 24, 2013
The same day, YouTuber Daimondo uploaded a video featuring a 3D-rendered model of the scepter in the sandbox game Garry’s Mod (shown below, left). Also on November 24th, the My Little Pony blog Equestria Daily published an article about the overnight popularity surrounding the scepter, providing links to Garry’s Mod and Team Fortress 2 3D models of the scepter and a newly-created “Ask Twilight Scepter” Tumblr blog. On November 25th, YouTuber MrMental4 uploaded a parody of the Leekspin animation featuring Twilight Sparkle and the scepter (shown below, right).
On the same day, MLP Forums member Bunny submitted a thread about the scepter, noting its prevalence on the My Little Pony-related image hosting site Derpibooru. Also on November 25th, Redditor Scottfra submitted an animated GIF of a dead horse being beaten with the scepter (shown below) to the /r/mylittlepony subreddit, which was initially uploaded to Know Your Meme by user Tyler Bowman a day earlier, gaining over 520 up votes and 35 comments in the first 16 hours.
Additional examples can be found on Derpibooru under the tag “twilight scepter”.