The meme "Twilight Milked Too hard" was submitted in a discord 4chan Brony server in response of some one posting a lewd photo in the general channels instead of the correct channel. it had an AI generated Twilight Sparkle looking like a cow and the text saying she milked too hard. this can be humorous because milking is a slang for masterbating and Twilight turning into a cow because of a lewd picture is subverting expectation be cause she "milked" too hard that she turned into a cow