Originally found on an episode of Squid Billies. Turned into a small time meme some time this year. No names or credibile information on who made it quite yet. Except those of course who created the show.
Found on a few forums but not sure on which one it originally came from..Until more information is found out more credibility may be written down. Season 2 Episode 2 of Squid Billies. Episode name being Butt Trouble.

Rusty ends up laying eggs out his butt and Earlie worries about his son being a bitch. So Earlie tries coming up with ways to make his son a man. Taking Rusty out to the woods where they dress up in fruit costumes and wait for a deer. No deer coming but a squirrel, so Earlie tries to make Rusty shoot it to become a man. Rusty averts his rifle and accidentally shoots his Granny.

Squidbillies is an animated television series about the Cuylers, an impoverished family of anthropomorphic hillbilly squids living in the Appalachian region of North Georgia's mountains. The show is produced by Williams Street Studios for the Adult Swim programming block of Cartoon Network. It is written by Dave Willis, of Aqua Teen Hunger Force fame, and Jim Fortier, previously of The Brak Show, both of whom worked on the Adult Swim series Space Ghost Coast to Coast.^[1] The animation is done by Radical Axis, with background design by Ben Prisk.

Squidbillies follows the exploits of the Cuyler family and their interactions with the local populace, which usually results in a fair amount of destruction, mutilation, and death. The Cuylers are given essentially free rein and protected from the consequences of their actions whenever possible by the Sheriff, as they are said to be the last, twisted remnants of a Federally-protected endangered species, the "Appalachian Mud Squid". Themes include the family's overt racism against white people (Chalkies), Early's dalliances with Krystal, their sketchy friendship with the Sheriff, run-ins with celebrities, their crushing poverty, and the machinations of corporate executive Dan Halen. The series has very little continuity: storyline progression and character development are present to an extent, but characters are frequently killed or seriously injured and return unharmed--often without explanation, and sometimes in the very same episode, forcing viewers to suspend their disbelief. They live in the Southern Appalachian Mountains located in Northern Georgia.
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